Novel and Important Research


28th of November 2022

Falsely low phosphatidylethanol may be associated withbiomarkers of haemolytic disease

In their latest research article Arving et al. discuss that falsely lower or even negative phosphatidylethanol (PEth) levels may theoretically be seen in patients with haemolytic diseases.

The article has been published in the Journal Basic and Clinical Pharmacology and Toxicology.


24th of November 2022

Haematocrit correction of whole blood phosphatidylethanol (PEth) concentrations to estimate erythrocyte PEth concentrations: sensitivity, specificity and influence on test utility

In their latest Technical Note White et al. investigated whether erythrocyte PEth might be validly calculated by haematocrit correction of a whole blood PEth measurement. They concluded that haematocrit-correction of whole blood PEth concentrations provides an alternative to preparation of washed erythrocytes.

The article has been published in the Journal of Analytical Toxicology.


7th of November 2022

Development and validation of seven phosphatidylethanol-homologues in dried blood spots including preliminary results after excessive use of an ethanol-based hand sanitizer

Herzog et al. from the FTC Munich published a study investigating the impact of PEth formation during the excessive use of an ethanol-based hand sanitizer. They conclude, that in cases of regular alcohol consumption, PEth-homologues are not likely to be further influenced by hand sanitizers.

The study has been published in the Journal of Analytical Toxicology.


24th of October 2022

PEth 16:0/18:1 and 16:0/18:2 after consumption of low doses of alcohol – A contribution to cutoff discussion

Aboutara et al. published a study investigating PEth 16:0/18:1 and PEth 16:0/18:2 in 75 participants who drank 20 g of alcohol on three consecutive evenings. They suggest that   PEth cut‐off concentrations should be lowered for better detection of consumption of low to medium amounts of alcohol. Furthermore, they found it advantageous to analyze both PEth 16:0/18:2 and 16:0/18:1. The study is published in the Journal Drug Testing and Analysis.


21st of October 2022

Can PEth be Detected with a Cutoff of 20 ng/mL after Single Alcohol Consumption?

Stöth et al. studied the amount of blood alcohol concentration that is needed in order to reach the 20 ng/mL cutoff for PEth 16:0/18:1 during a drinking study with twelve volunteers that have been abstinent for four weeks.

Despite drinking doses up to 0.58 g ethanol per kg body weight and reaching BACs of up to 0.63 g/kg, PEth 16:0/18:1 and PEth 16:0/18:2 could not be detected at or above the 20 ng/mL cutoff in any participant at any time after the drinking events. They conclude that after long-term abstinence the cutoff of 20 ng/ml for single alcohol consumption leading to blood alcohol concentrations up to 0.63 g/kg is not exceeded. The article is published in the Journal of Analytical Toxicology.


7th of September 2022

False Positive Results of Phosphatidylethanol (PEth) Quantitation in Dried Blood Spots (DBS): The Influence of Alcohol Vapors

Bashilov et al. from Moscow, Russia, investigated the influence of alcohol vapors regarding false positive results of phosphatidylethanol (PEth) quantitation in dried blood spots (DBS).

The article is available as open access publication by following this link.


10th May 2022

Validation of a liquid chromatography tandem mass spectrometry (LC-MS/MS) method for erythrocyte phosphatidylethanol revealing critical considerations for its use as a clinical biomarker

In this novel research paper, White et al. describe an assay for erythrocyte PEth 16:0/18:1. It is available in the Journal of Chromatography B.


10th May 2022

Positive blood phosphatidylethanol concentration is associated with unfavorable waitlist-related outcomes for patients medically appropriate for liver transplantation

A novel study This study examined the association of PEth results with liver transplantation waitlist-focused patient outcomes. It is available in the journal Alcoholism – Clinical and Experimental Research


9th May 2022

Availability of PEth testing is associated with reduced eligibility for liver transplant among patients with alcohol-related liver disease

This novel study investigated whether the availability of PEth testing impacted rates of liver transplant evaluation terminations and delistings. It is available in the journal Clinical Transplantation.


14th February 2022

Current evolutions, applications, and challenges of phosphatidylethanol analysis for clinical and forensic purposes

A novel overview about “current evolutions, applications, and challenges of phosphatidylethanol analysis for clinical and forensic purposes” is available now at WIREs Forensic Science. The aim of the overview is to lead the way for PEth harmonization together with the PEth in Mind conference in May 2022.


13th February 2022

Novel US-Patent for quantifying phosphatidylethanol from blood samples using gas chromatography mass spectrometry

In collaboration with the Loyola University of Chicago, James Vincent DeFrancesco (Arlington Heights, IL) and Majid Afshar (Chicago, IL) issued a novel patent for the determination of PEth using gas chromatography (patent number: 11085939).


25th January 2022

A positive test result for the alcohol biomarker phosphatidylethanol can still be used to underpin short-term abstinence

PEth-NET members Katleen van Uytfanghe and Christophe Stove just published about the “Set-up of a population-based model to verify alcohol abstinence via monitoring of the direct alcohol marker phosphatidylethanol 16:0/18:1” in the journal Addiction.


2nd December 2021

Phosphatidylethanol in patients with liver diseases of different etiologies: Analysis of six homologues and comparison with other alcohol markers

Nadine Aboutara et al. published novel research in Clinica Chimica Acta.

Highlights

•Sensitivity of uEtG, hEtG and CDT for drinking alcohol in the past 3 months was 29%

•PEth 16:0/18:1’s sensitivity was 53% for alcohol consumption in the past 3 months.

•For consumption of seven standard drinks per week PEth’s sensitivity was 92%.

•Quotients of some PEth-homologues were smaller if alcohol uptake was more recently.

•In 50% of the patients who stopped alcohol uptake 4 weeks prior, PEth was positive.


29th July 2021

Factors associated with phosphatidylethanol (PEth) sensitivity for detecting unhealthy alcohol use: An individual patient data meta-analysis

Judy Hahn et al. just published a meta-analysis in the Journal Alcoholism, Clinical & Experimental Research. They report, that age, sex, and the method of blood collection were not associated with PEth sensitivity. However, several biological factors (hemoglobin, BMI, liver fibrosis, and HIV status) were associated with PEth sensitivity. Race/ethnicity was associated with PEth sensitivity in some models. Overall they conclude, that researchers should consider adjusting analyses for these characteristics where possible.


27th May 2021

Comparison of the Diagnostic Value of Phosphatidylethanoland Carbohydrate-Deficient Transferrin

Alexander Arving et al. compare the diagnostic value of Phosphatidylethanol and Carbohydrate-Deficient Transferrin (CDT) as biomarkers of alcohol consumption in their latest article (available for free) published in Alcoholism: Clinical and Experimental Research.


20th April 2021

Hematocrit Corrected PEth Analysis

The article “Fully Automated Correction for the Hematocrit Bias of Non-Volumetric Dried Blood Spot Phosphatidylethanol Analysis” is now available in the journal Alcohol.

An explanatory video is available on youtube:


11th March 2021

Old but gold…?


16th February 2021

PEth post sampling formation from ethanol

In their latest publication, Olof Beck, Maria Mellring, Christian Löwbeer, Sabina Seferaj & Anders Helander investigated the post-sampling formation of PEth in various devices for blood microsampling.

they found that:

“with three commercial devices for volumetric dried blood microsampling, which is considered important for use in test applications needing precise substance quantification [4142], post-sampling formation of PEth from ethanol was observed with all of them (Mitra, Capitainer, Hemaxis), albeit to varying degrees, upon drying and storage at room temperature.”

They investigated that pretreatment of the DBS sampling substrate with an inhibitor (NaVO3) can stop the formation of PEth

No PEth formation was observed when blood spiked with ethanol at a physiologically relevant concentration (2 g/L) was added to Protein Saver card filters.

The article is published in Analytical and Bioanalytical Chemistry and is freely available by following this link.


4th January 2021

Quantitation of phosphatidylethanol in dried blood after volumetric absorptive microsampling

In their latest publication, Katleen Van Uytfanghe, Maria Del Mar Ramirez Fernandez, Aurelie De Vos, Sarah MR. Wille and Christophe Stove present the application of PEth analysis in Belgium, at the Ghent University’s Laboratory of Toxicology and the National Institute of Criminalistics and Criminology .

Congratulations on the successful initiation of a Belgian network for quantifying phosphatidylethanol 16:0/18:1.

The article is published in Talanta and is freely available by following this link.


8th December 2020

Protocol for PEth DBS Analysis

A protocol for the analysis of PEth in DBS (manually or automated) has been published by Springer-Nature in the Journal “Nature Protocols”.

The article is freely available follwing this link:

https://rdcu.be/cbOJ5


Highlight, 6th November 2020

The Importance of Regioisomeric Purity in PEth Reference Material

In their publication, Marc Luginbühl, Reuben S E Young, Frederike Stöth, Wolfgang Weinmann, Stephen J Blanksby, and Stefan Gaugler show the importance of the right choice of reference material for reliable PEth research.

“The results suggest caution in ensuring the regioisomeric composition of reference materials are well-matched with the authentic blood samples.”

The article is published in the Journal of Analytical Toxicology and can be found following this link: https://pubmed.ncbi.nlm.nih.gov/32248226/


4th November 2020

Stability of Phosphatidylethanol 16:0/18:1 in Freshly Drawn, Authentic Samples from Healthy Volunteers

In their latest publication, Ragnhild Bergene Skråstad, Olav Spigset, Trond Oskar Aamo and Trine Andreassen challenge current knowledge about PEth storage and stability.

Within their novel publication they state “PEth 16:0/18:1 is stable for 28 days irrespective of storage temperature”

The article is published in the Journal of Analytical Toxicology and can be found following this link: https://pubmed.ncbi.nlm.nih.gov/32754728/


6th October 2020

Testing venous CDT or capillary PEth with concurrent EtG and EtPa hair tests to assess historical and recent alcohol use

In their latest publication, L. Tsanaclis, M. Davies, S. Bevan, J. Nutt, K. Bagley and J. Wicks compare direct (EtG, FAEE, PEth) and indirect (CDT) alcohol biomarkers either measured in hair or blood.

The results of the study “substantiate the case for using hair EtG in conjunction with a PEth test, rather than CDT test, for efficient monitoring of recent and historical alcohol consumption.”

The article is published in Drug Testing and Analysis and can be found following this link: https://doi.org/10.1002/dta.2939